Contribution of the Leukocyte Adherence Inhibition Test to the Diagnosis of Non–IgE-mediated Immunoreactivity against Candida albicans in Patients with Atopic Dermatitis
Published: 2023-11-14
Page: 268-276
Issue: 2023 - Volume 6 [Issue 1]
Celso Eduardo Olivier *
Instituto Alergoimuno de Americana, Brazil.
Daiana Guedes Pinto
Instituto Alergoimuno de Americana, Brazil.
Ana Paula Monezzi Teixeira
Instituto Alergoimuno de Americana, Brazil.
Jhéssica Letícia Santos Santana
Instituto Alergoimuno de Americana, Brazil.
Raquel Acácia Pereira Gonçalves Santos
Instituto Alergoimuno de Americana, Brazil.
Regiane Patussi Santos Lima
Lavoisier’s Laboratories, São Paulo, Brazil.
Everton Salgado Monteiro
Department of Allergy and Immunopathology, Faculty of Medicine, São Paulo University, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Aims: To evaluate the potential of the Leukocyte Adherence Inhibition Test (LAIT) to discriminate Non–IgE-mediated immunoreactivity against Candida albicans in Atopic Dermatitis () patients with clinical suspicion of hypersensitivity reactions to fungal allergens.
Study Design: We retrospectively examined the medical charts of 100 patients diagnosed with with clinical suspicion of Non–IgE-mediated fungal hypersensitivity who were investigated with an ex vivo challenge monitored by LAIT against an extract of Candida albicans.
Place and Duration of Study: Instituto Alergoimuno de Americana – São Paulo – Brazil – between January 2018 and October 2023.
Methodology: The percentage of Leukocyte Adherence Inhibition (LAI) promoted by the ex vivo challenges with C. albicans extract was distributed in ranges through a cascade distribution chart to outline the variability of the results.
Results: The mean LAI was 41.5%; SD 29.7%, ranging from 0% to 100%; mode = 0% (appeared 17 times). A wide distribution of LAI results suggested that some patients had immunoreactivity against the Candida albicans allergens while tolerant ones did not.
Conclusion: Our preliminary results support that the LAIT performed with Candida albicans may differentiate diverse degrees of ex vivo immunoreactivity against this airborne allergen in allergic patients.
Keywords: Allergy, Candida albicans, atopic dermatitis, diagnosis, hypersensitivity, leukocyte adherence inhibition test, non–IgE-mediated immunoreactivity