Contribution of the Leukocyte Adherence Inhibition Test to the Diagnosis of Innate Non–IgE-mediated Immunoreactivity against Alternaria alternata
Published: 2023-10-03
Page: 243-251
Issue: 2023 - Volume 6 [Issue 1]
Celso Eduardo Olivier *
Instituto Alergoimuno de Americana, Brazil.
Daiana Guedes Pinto
Instituto Alergoimuno de Americana, Brazil.
Ana Paula Monezzi Teixeira
Instituto Alergoimuno de Americana, Brazil.
Jhéssica Letícia Santos Santana
Instituto Alergoimuno de Americana, Brazil.
Raquel Acácia Pereira Gonçalves Santos
Instituto Alergoimuno de Americana, Brazil.
Regiane Patussi Santos Lima
Lavoisier’s Laboratories, São Paulo, Brazil.
Everton Salgado Monteiro
Department of Allergy and Immunopathology, Faculty of Medicine, São Paulo University, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Aims: To evaluate the potential of the Leukocyte Adherence Inhibition Test (LAIT) to discriminate innate immunoreactivity against Alternaria alternata in patients with clinical suspicion of allergic reaction to fungal allergens.
Study Design: We retrospectively examined the medical charts of 100 patients diagnosed with Allergic Rhinitis and/or Asthma with clinical suspicion of fungal hypersensitivity who were investigated with an ex vivo challenge monitored by LAIT against the extract of A. alternata.
Place and Duration of Study: Instituto Alergoimuno de Americana – São Paulo – Brazil – between January 2018 and August 2023.
Methodology The percentage of Leukocyte Adherence Inhibition (LAI) promoted by the ex vivo challenges with A. alternata extract was distributed in ranges through a cascade distribution chart to outline the variability of the results.
Results: The mean LAI was 50%; SD 22,2%, ranging from 0% to 94%; mode = 58% (appeared five times). There was a wide range of distribution of LAI results, suggesting that some patients had immunoreactivity against the A. alternata allergens while others did not.
Conclusion: Our preliminary results support that the LAIT performed with A. alternata may differentiate diverse degrees of ex vivo immunoreactivity against this airborne antigen in allergic patients.
Keywords: Allergy, Alternaria alternata, asthma, diagnosis, hypersensitivity, leukocyte adherence inhibition test, Non–IgE-mediated immunoreactivity, rhinitis
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