Differential Leukocyte Ratios in Women with Unexplained Recurrent Implantation Failures
Asian Journal of Immunology, Volume 5, Issue 1,
Background: Unexplained recurrent implantation failures are currently a public health problem especially in the Sub Saharan Africa. It is a medical condition that has been attributed to many factors including low-grade chronic inflammation. Differential leukocyte ratios are efficient markers of low-grade chronic inflammation. There is a paucity of data on the levels of differential leukocyte ratios in women with unexplained recurrent implantation failures in the Enugu population.
Objectives: The present study aims to determine the levels of the differential leukocyte ratios involving the Neutrophil Lymphocyte Ratio(NLR), Monocyte Lymphocyte Ratio(MLR), Basophil Lymphocyte Ratio(BLR) and the Eosinophil Lymphocyte Ratio(ELR) in women with unexplained recurrent implantation failures and healthy controls.
Materials and Methods: This was a cross-sectional case controlled study. A total of 80 subjects involving 40 cases and 40 controls aged 20-35 years were recruited for the study. Blood samples were collected from the subjects by venipuncture. The differential leukocyte ratios were calculated manually from their fully blood count results determined with the Mindray 530BC automated analyzer, Mindray, Japan. The data were analyzed with SPSS version 23 the using Student T- test and presented as the mean ± standard deviation from the mean and a p-value <0.05 considered was significant.
Results: The results revealed significant increase in the NLR(2.99 ± 0.43), ELR(4.66 ± 0.9),and MLR(1.91 ± 2.06) in the women with unexplained recurrent implantation failures compared to NLR(1.80 ± 0.19), ELR(2.86 ± 0.40) and MLR(0.71 ± 1.41) respectively in the healthy women.
Conclusion: This finding supports the claim for a low grade chronic inflammation in women with unexplained recurrent implantation failures.
- Differential leukocyte ratios
- low-grade chronic inflammation
- unexplained recurrent reproductive failures
- assisted reproductive therapy
- window of implantation
How to Cite
Timeva T, Atanas S, Stanimir K. Recurrent implantation failure:the role of endometrium. Journal of Reproductive Infertility. 2014;15:173-183.
Siritidas S, Paraskevi V, Nikos B, Aspasia L, Zoe I, Stefano B, Charalampos C. Micro RNA in assisted reproduction and their potential role in IVF failure. In vivo. 2016 29:169-175.
RefaatB.Role of activins in embryo implantation and diagnosis of ectopic pregnancy: A review. Reproductive Biology and Endocrinology. 2014;12:16
Batsu E, Mehmet M, Cenk Y, Ozlem D, Asli A, Cem C, et al. Role of mucin 1 and glycodelin A in recurrent implantation failure. Fertility and Sterility. 2015;103: 1059-1064.
Choi H, Chung T, Park M, Kim H, You S, Myeong S, ET AL. Benzoic acid enhances embryo implantation through LIF- dependent of integrin Bβ3 and Vβ5. Journal of Microbiology Biotechnology. 2017;27:668-677.
Comins A, Segovia A, Prado N, Fuente L, Alonso J, Ramon S. Evidence-based update: Immunological evaluation of recurrent implantation failure. Reproductive Immunology Open Access. 2016; 1:1-8.
Koot YEM, Hoof S, Boomsma MC, Leenen D, Koerkamp M, Goddijn M, et al. An endometrial gene expression signature accurately predicts recurrent implantation failure after IVF. Scientific Report. 2016; 6:1-12
Khalife D, Ghazeeri G. Recurrent implantation failure and low molecular weight heparin. Open Journal of Obstetrics and Gynecology. 2018; 8:148-162.
Bakish R, Jha B, Prakash R. The impact of endometrial scratching on the outcome of in vitro fertilization cycles: A retrospective study. Fertility Science Research. 2015; 3:80-86.
SadeghiM.The 40th Anniversary of IVF? Journal of Reproductive Infertility. 2018; 19:67-68.
Cekici Y, Yilmaz M, Secen O. New inflammatory indicators: Association of high eosinophil -to- lymphocyte ratio and low lymphocyte-to-monocyte ratio with smoking. Journal of International Medical Research. 2019;49(9):4292-4303
Ndulue DC, Ayadiuno RU, Mozie AT, Ogbu CI. Spatial variation in the level of awareness and application of climate change policies and laws in Enugu State, South East Nigeria. Psychology and Education. 2021;58(2):6466-6471.
Shonde-Adebola KB, Shokunbi WA, Adebola MB. Prevalence of von Willebrand disease among Nigerian youths in Ibadan, south-western, Nigeria. Open Access Journal. 2021;8(e7789):1-8.
Akinsegun A, Olusola DA, Sarah J-O, Olajumoke O, Adewumi A, Majeed O, et al. Mean Platelet Volume and Platelet Counts in Type 2 Diabetes Mellitus on treatment and Non –Diabetes Mellitus controls. Pan African Medical Journal. 2014;18(42):1- 5
Kslisic A, Kotur-Stevuljevic J, Ninic A. White blood cell counts, its subsets and their indexes in type 2 diabetes mellitus. Archives of Pharmacy. 2022;72:193- 199.
Cemil OS, Aydin MF. Are neutrophil to lymphocyte ratio and platelet to lymphocyte ratio clinically useful for the prediction of early pregnancy loss? Ginekology Polska. 2020;91:524-527
Ashoush SA. Hematological inflammatory biomarkers affecting the success rate of in vitro fertilization among cases of unexplained infertility. Egypt Journal of Fertility and Sterility. 2019;23(1):1-9.
D’ Angelo G. Inflammation and coagulation: A continuum between coagulation activation and prothrombotic state. Journal of Blood Disorders. 2015; 2(1):1023.
Dankova IV, Aleksandrovna MO, Borisovna TT, Anatolyevna PL, Olegovich MD, Valeryevna CO, Evgencyevich RN. Genetic and hemostasiological predictors of IVF pregnancy. Gynecology Endocrinology. 2017;33(S1):32-35.
Gerotziafas GT, Dreden PV, d’ Argent EM, Lefkou E, Grusse M, Comtet M, et al. Impact of blood hypercoagulability on in vitro fertilization outcomes: A propective longitudinal observational study. Thrombosis Journal. 2017;9:1-11.
Abstract View: 119 times
PDF Download: 0 times