Asian Journal of Immunology

Activity Guided Isolation of Phytoconstituents of Moringa oleifera. Lam Leaves to Study their In-vitro Free Radical Scavenging Activity

Devaraj V. C. *

Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Hyderabad, India.

B. Gopala Krishna

Department of Pharmacognosy, RR College of Pharmacy, Bangalore, India.

*Author to whom correspondence should be addressed.

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Abstract

Aim: To isolate the antioxidative components of Moringa oleifera. L leaves by screening their free radical scavenging potential.

Methodology: The leaves of Moringa oleifera. L were dried in the shade, followed by grinding and cold macerating with 95% alcohol. The crude alcoholic extract was then fractionated with organic solvents like ether, chloroform, and ethyl acetate. The in-vitro free radical scavenging potential was assessed by α the α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and Nitric oxide scavenging methods.

Results: Among the different leaf fractions of Moringa oleifera assessed, the ethylacetate fraction exhibited potential free radical scavenging potentiality, hence these fractions were further progressed for fractionation and refining using advanced chromatographic techniques. The sequential fractionation and purification resulted in the isolation of three components which were structurally characterized and identified employing advanced analytical techniques like UV, IR, Mass spectroscopy, and NMR spectroscopic methods. In comparison with the available literature data, the isolated compounds were named quercetin (1), kaempferol (2), and quercetin-3-O-rutinoside (3). The compounds isolated from the leaves of Moringa oleifera exhibited potential free radical scavenging activity on testing for their antioxidant property.

Conclusion: The compounds isolated from the Moringa oleifera leaves exhibited a strong free radical scavenging potential proving the antioxidant property of the plant.

Keywords: Moringa oleifera Lam, antioxidant activity, DPPH, nitric oxide

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References

Bahar Mummed, Ashebr Abraha, Teka Feyera, Adugna Nigusse, Solomon Assefa. In-vitro antibacterial activity of selected medicinal plants in the Traditional treatment of skin and wound infections in Eastern Ethiopia. Biomed. Res. Int. 2018; 1862401:1-8.

Pallavi S, Ambuj BJ, Rama SD, Mohammad P. Reactive oxygen species, oxidative damage, and anti-oxidative defense mechanism in plants under stressful conditions. J Botany 2012;2012: 1–26.

HSR Michael, NB Mohammed, S Ponnusamy, WE Gnanaraj. A Folk Medicine: Passiflora incarnata L – Phytochemical Profile with antioxidant potency. Turk J Pharm Sci. 2022;19(3): 287-292.

Nan Wu, Kuang Fu, Yu-Jie Fu, Yuan GZ, Fang RC, Yung HC, Xiao LL, Kong Y, Wei L, Cheng BG. Antioxidant activities of extracts and main components of Pigeonpea [Cajanus cajan (L.) Millsp] leaves. Molecules. 2009;14:1032–1043.

Riaz MU, Raza MA, Saeed A, Ahmed M, Hussain T. Variations in Morphological Characters and Antioxidant Potential of Different Plant Parts of Four Ziziphus Mill. Species from the Cholistan. Plants (Basel). 2021;10(12):2734.

Sainan Li Yujing Xia Kan Chen Jingjing Li Tong Liu Fan Wang Jie Lu Yingqun Zhou Chuanyong Guo. Epigallocatechin-3-gallate attenuates apoptosis and autophagy in concanavalin A-induced hepatitis by inhibiting BNIP3. Dru. Des. Dev. Ther. 2016;10: 631–647.

Maryam A, Mohammad-Reza D, Maryam K, Rafi A, Saeed T. SDE-prepared oil analysis and evaluation of antioxidant and antibacterial potentials of Varthemia persica DC. Pharma Sci. 2014;20:70-76.

P Trakoolthong, N Ditthawuttikul, BS Sivamaruthi, S Sirilun, W Rungseevijitprapa, S Peerajan, C Chaiyasut. Antioxidant and 5α-Reductase Inhibitory Activity of Momordica charantia Extract, and Development and Characterization of Microemulsion. Appl. Sci. 2022;12:4410.

R Imperatore, B Fronte, D Scicchitano, G Orso, M Marchese, M Paolucci. Dietary supplementation with a blend of hydrolyzable and condensed Tannins ameliorates diet-induced intestinal Inflammation in Zebrafish (Danio rerio). Animals. 2023;13:167.

Padmanabhan P, Jangle SN. Evaluation of DPPH radical scavenging activity and reducing power of four selected medicinal plants and their combinations. Inter J Pharm Sci Drug Res. 2012;4:143-146.

Ganesh NS, Susheel KD, Nitin S, Jyotsana S. Anti-inflammatory Activity and Total Flavonoid Content of Aegle marmelos Seeds. Int J Pharm Sci Drug Res. 2011;3:214-218.

Jelena T, Anamaria B, Mirela BL, Cvijeta JB, Ana KM, Monika B. Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells. Antioxidants. 2020;9(5):453.

Biswas SK, Chowdhury A, Das J, Roy A, Hosen SM. Pharmacological potentials of Moringa oleifera Lam: A review. Int J Pharm Sci Res. 2012;3:305–310.

Munguia AL, Romero YH, Chaverri JP, Molina AM, Regla I, Martinez A, Castillo E. Phenylpropanoid glycoside analogues: Enzymatic synthesis, antioxidant activity and theoretical study of their free radical scavenger mechanism. PLoS One. 2011; 6:1–9.

Akiri SVCR1, Sareddy GR2, Phanithi PB, Attipalli RR. The antioxidant and antiproliferative activities of methanolic extracts from Njavara rice bran. BMC Compl Altern Med. 2010;10:1–9.

Zhang L, Li BG, Tian FR. Studies on flavonoids of Artemisia anomala S. Moore. Chin Pharm J 2010;45:104–107.

Gitin L, Dinica R, Parnavel R. The influence of extraction method on the apparent content of bioactive compounds in Romanian Allium spp. Leaves. Not Bot Horti Agrobo. 2012;40:93–97.

Marzouk MS, Moharram FA, El Dib RA, El-Shenawy SM, Tawfike AF. Polyphenolic profile and bioactivity study of Oenothera speciosa Nutt. aerial parts. Molecules. 2009;14:1456–1467.

Said A, Huefner A, El-Sayed AAT, Fawzy G. Phenolic compounds from seeds of Zizyphus spina-christi. IUFS J Biol 2011; 70:39–43.

Q. M. Chen, MR Fu, FL Yue, YY Cheng. Effect of superfine grinding on physicochemical properties, antioxidant activity and phenolic content of Red Rice (Oryza sativa L). Food. Nut. Sci. 2015; 6:1277-1284.

Marcello Nicoletti. The Antioxidant Activity of Mistletoes (Viscum album and Other Species). Plants. 2023;12:2707.

Iram I, Polrat W, Fatima S, Bushra N, Muqeet W, Muhammad FL, et al. Plant polyphenols and their potential benefits on cardiovascular Health: A Review. Molecules 2023;28: 6403.

Miller MJ, Sadowska–krowicka H, Chotinaruemol S, Kakkis JL, Clark DA. Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Therap. 1993;264:11–16.

Guo X, Wang WP, Ko JK, Cho CH. Involvement of neutrophils and free radicals in the potentiating effects of passive cigarette smoking on inflammatory bowel disease in rats. Gasteroenterol. 1999;117:884–892.

Aysooda H, Omid S, Akram NB, Sepideh S, Gabriel S. Ahmad E. Immunomodulatory effects of flavonoids: Possible induction of T CD4+ Regulatory Cells through suppression of mTOR Pathway signaling activity. Front. Immunol. 2019;10:Article- 51.