Distribution of FCR Polymorphism in Children Endemic Population of Burkina and Effect of this Polymorphism on IgG and his Subclass Production

Main Article Content

Mariama Kaba Combasseré-Cherif
Fatimata Thiombiano
Boubacar Maiga
Daniel Amoako-Sakyi
Romaric Nebnoma Tiendrébeogo
Amagana Dolo
Sodiomon B. Sirima
Issa Nébié
Marita Troye-Blomberg


Aims: Malaria is one of disease which caused many deaths every year. Many studies try to understand why since it apparition, malaria is still endemic in certain regions. It appear that some FCR polymorphism may impact on susceptibility to malaria. The present study aimed to determine the distribution of certain FCR polymorphism and their effect on antibodies production.

Study Design: Two cross-sectional surveys were carried out at Saponé.

Place and Duration of Study: Sampling was done at Saponé during low (January 2007) and high (September 2007) malaria transmissions seasons and laboratories activities were done at the department of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Sweden. Methodology: During each cross-sectional survey, 5 ml of venous blood in a tube containing EDTA was collected from each child for antibodies assessment by ELISA and FCR genotyping by PCR. Thick and thin blood films were also prepared from finger prick for microscopy diagnosis of malaria.

Results: 622 children from Mossi tribes participated in this study. Three mutants allele were present in pupation with Frequencies of the mutant alleles of FCGR2C (rs3933769), FCGR3A (rs396991) and FCGR2B (rs1050519) 15.43%, 66.24% and 37.94% respectively. Regarding the SNPs, they are contribute to malaria pathogenesis. Carriers of the mutant allele of FcγRIIB (rs1050519) harboured more parasites than the parasites harboured by non-carriers (P=.002). However, the mutant alleles of FCγRIIC (rs3933769) and FCγRIIIA (rs396991) harboured less parasite than wild alleles (P=.001) for all of them.  SNPs are associated to total IgG responses. The   FCγRIIB (rs 1050519) SNP (permutation of G to T) was negatively associated with IgG responses.  Children with allele GG (Wild type) produced more antibodies against MSP3 than children with GT (Heterozygous) and TT (Mutant). The difference was statically significant (P= 0.05). However the FcRIIB mutation was positively associated with cytophilic IgG production against MSP2B, GLURP R0 and GLURP R2. The difference was statistically significant for MSP2A (P<0.001).

Conclusion: FCR2b, FCR2c and FCR3a are present in or study population. Malaria antibodies seem to be affected by FCR mutations.

Polymorphism, FCR mutations, malaria, P. falciparum, children.

Article Details

How to Cite
Combasseré-Cherif, M. K., Thiombiano, F., Maiga, B., Amoako-Sakyi, D., Tiendrébeogo, R. N., Dolo, A., Sirima, S. B., Nébié, I., & Troye-Blomberg, M. (2021). Distribution of FCR Polymorphism in Children Endemic Population of Burkina and Effect of this Polymorphism on IgG and his Subclass Production. Asian Journal of Immunology, 4(4). Retrieved from https://journalaji.com/index.php/AJI/article/view/30144
Original Research Article


(WHO), W.H.O. WHO global database on insecticide resistance in malaria vectors; 2020.

Pwalia R, et al., High insecticide resistance intensity of anopheles gambiae (s.l.) and low efficacy of pyrethroid LLINs in Accra, Ghana. Parasit Vectors, 2019;12(1):299.

Adu, B et al. Antibody levels against GLURP R2, MSP1 block 2 hybrid and AS202.11 and the risk of malaria in children living in hyperendemic (Burkina Faso) and hypo-endemic (Ghana) areas. Malar J, 2016;15:123.

Cherif MK et al. Antibody responses to P. falciparum blood stage antigens and incidence of clinical malaria in children living in endemic area in Burkina Faso. BMC Res Notes, 2017;10(1):472.

Dolo A et al. Difference in susceptibility to malaria between two sympatric ethnic groups in Mali. Am J Trop Med Hyg. 2005. 72(3):243-8.

Giha HA et al. Antigen-specific influence of GM/KM allotypes on IgG isotypes and association of GM allotypes with suscep tibility to Plasmodium falciparum malaria. Malar J, 2009;8:306.

Israelsson E et al. Marked differences in CRP genotype frequencies between the Fulani and sympatric ethnic groups in Africa. Malar J, 2009;8:136.

Modiano D et al., Different response to Plasmodium falciparum malaria in west African sympatric ethnic groups. Proc Natl Acad Sci USA. 1996;93(23):13206-11.

Nasr A, et al. FcgammaRIIa (CD32) polymorphism and anti-malarial IgG sub class pattern among Fulani and sympatric ethnic groups living in eastern Sudan. Malar J. 2009;8:43.

Nebie I et al. Humoral responses to defined malaria antigens in children living since birth under insecticide treated curtains in Burkina Faso. Acta Trop. 2003; 88(1):17-25.

Nebie I et al. Humoral responses to Plasmodium falciparum blood-stage antigens and association with incidence of clinical malaria in children living in an area of seasonal malaria transmission in Burkina Faso, West Africa. Infect Immun, 2008;76(2):759-66.

Nebie I et al. Do antibody responses to malaria vaccine candidates influenced by the level of malaria transmission protect from malaria? Trop Med Int Health. 2008; 13(2):229-37.

Perez LG et al. Utilization of immunoglo bulin G Fc receptors by human immuno deficiency virus type 1: A specific role for antibodies against the membrane-proximal external region of gp41. J Virol. 2009; 83 (15):7397-410.

Israelsson E et al. Differences in Fcgamma receptor IIa genotypes and IgG subclass pattern of anti-malarial antibodies between sympatric ethnic groups in Mali. Malar J, 2008;7:175.

Clatworthy MR et al. Systemic lupus erythematosus-associated defects in the inhibitory receptor FcgammaRIIb reduce susceptibility to malaria. Proc Natl Acad Sci USA. 2007;104(17):7169-74.

Willcocks LC et al. A defunctioning polymorphism in FCGR2B is associated with protection against malaria but susceptibility to systemic lupus erythe matosus. Proc Natl Acad Sci USA. 2010. 107(17):7881-5.

Cherif M et al. Distribution of FcgammaR gene polymorphisms among two sympatric populations in Mali: Differing allele frequen cies, associations with malariometric indices and implications for genetic susce ptibility to malaria. Malar J. 2016;15:29.

Cherif MK et al. Fcgamma RIIa poly morphism and anti-malaria-specific IgG and IgG subclass responses in populations differing in susceptibility to malaria in Bur kina Faso. Scand J Immunol. 2012;75(6): 606-13.

Diarra A et al. Antibodies to malaria vaccine candidates are associated with chloroquine or sulphadoxine/ pyrimetha mine treatment efficacy in children in an endemic area of Burkina Faso. Malar J, 2012;11:79.

Dodoo D et al. Cohort study of the association of antibody levels to AMA1, MSP119, MSP3 and GLURP with protection from clinical malaria in Ghanaian children. Malar J. 2008;7:142.

Tongren JE et al. Target antigen, age, and duration of antigen exposure indepen dently regulate immunoglobulin G subclass switching in malaria. Infect Immun, 2006; 74(1):257-64.

Bowles JA, Weiner GJ. CD16 polymorphisms and NK activation induced by monoclonal antibody-coated target cells J Immunol. Methods. 2005;304:88–99.

Koene HR et al. Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 1997;90:1109–14.

Wu J et al. A novel polymorphism of FcγRIIIa (CD16) alters receptor function and predisposes to autoimmune disease. J Clin Invest. 1997;100:1059–1070.

Congy-Jolivet N et al. Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype. Cancer Res. 2008;68:976–80.

TL, Foster CB, Zhu S, Venzon D, Stein berg SM, Wyvill K, et al. Variant genotypes of FcgammaRIIIA influence the develop ment of Kaposi’s sarcoma in HIV-infected men. Blood. 2000;95:2386–90.

Radstake TRDJ, Petit E, Pierlot C, van de Putte LBA, Cornelis F, Barrera P. Role of Fcgamma receptors IIA, IIIA, and IIIB in susceptibility to rheumatoid arthritis. J Rheumatol. 2003;30:926–33.

Nishio M, Endo T, Fujimoto K, Yamamoto S, Obara M, Yamaguchi K, et al. FCGR3A-158V/F polymorphism may correlate with the levels of immunoglobulin in patients with non-Hodgkin’s lymphoma after rituxi mab treatment as an adjuvant to autolo gous stem cell transplantat