Contribution of Leukocyte Adherence Inhibition Test in the Evaluation of Non–IgE-mediated Immunoreactivity against Peanut Proteins in Children and Adults with Atopic Dermatitis

Celso Eduardo Olivier *

Instituto Alergoimuno de Americana, Brazil.

Daiana Guedes Pinto

Instituto Alergoimuno de Americana, Brazil.

Ana Paula Monezzi Teixeira

Instituto Alergoimuno de Americana, Brazil.

Jhéssica Letícia Santos Santana

Instituto Alergoimuno de Americana, Brazil.

Raquel Acácia Pereira Gonçalves Santos

Instituto Alergoimuno de Americana, Brazil.

Regiane Patussi Santos Lima

Lavoisier’s laboratories , São Paulo, Brazil.

*Author to whom correspondence should be addressed.


Abstract

Aim: To evaluate the potential of the Leukocyte Adherence Inhibition Test (LAIT) to evaluate immunoreactivity against peanut proteins in patients with a clinical diagnosis of non–IgE-mediated Atopic Dermatitis (AD).

Study Design: We retrospectively examined the medical charts of a population of 51 children (0 to 17 years) and 275 adults (18 to 93 years) diagnosed with non–IgE-mediated AD who were investigated with an ex vivo challenge monitored by LAIT against peanut proteins.

Place and Duration of Study: Instituto Alergoimuno de Americana – São Paulo – Brazil – between January 2018 and February 2024.

Methodology: The percentage of Leukocyte Adherence Inhibition (LAI) promoted by the ex vivo challenges with peanut proteins was distributed in ranges through a cascade distribution chart to outline the variability of the results.

Results: In the child cohort, the LAI mean was 48.5%; the median was 52%; SD 27.6%; ranging from 0% to 95%; modes = 0% and 63% (each appeared four times). There was a normal wide range of distribution of LAI results, as outlined by the cascade distribution chart. In the adult cohort, the LAI mean was 43.8%; the median was 46%; SD 28.6%; ranging from 0% to 100%; modes = 0% (appeared 45 times). There was a normal wide range of distribution of LAI results, as outlined by the cascade distribution chart.

Conclusion: Our preliminary results support the idea that the LAIT performed with peanut extract may differentiate diverse degrees of ex vivo non–IgE-mediated immunoreactivity against peanut allergens in non–IgE-mediated AD patients, suggesting a potential contribution of LAIT in the stratification of endotypes and the phenotypic classification of non–IgE-mediated AD patients.

Keywords: Adults, allergy, atopic dermatitis, children, diagnosis, endotypes, food allergy, hypersensitivity, leukocyte adherence inhibition test, non–IgE-mediated immunoreactivity, peanut allergy, phenotypes, predictive biomarker


How to Cite

Olivier, C. E., Pinto , D. G., Teixeira, A. P. M., Santana, J. L. S., Santos, R. A. P. G., & Lima , R. P. S. (2024). Contribution of Leukocyte Adherence Inhibition Test in the Evaluation of Non–IgE-mediated Immunoreactivity against Peanut Proteins in Children and Adults with Atopic Dermatitis. Asian Journal of Immunology, 7(1), 55–62. Retrieved from https://journalaji.com/index.php/AJI/article/view/129

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